Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 Å
نویسندگان
چکیده
منابع مشابه
Crystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 A resolution.
The MunI restriction enzyme recognizes the palindromic hexanucleotide sequence C/AATTG (the '/' indicates the cleavage site). The crystal structure of its active site mutant D83A bound to cognate DNA has been determined at 1.7 A resolution. Base-specific contacts between MunI and DNA occur exclusively in the major groove. While DNA-binding sites of most other restriction enzymes are comprised o...
متن کاملCrystal structure of MunI restriction endonuclease in complex with cognate DNA at 1.7 resolution
The MunI restriction enzyme recognizes the palindromic hexanucleotide sequence C/AATTG (the ‘/’ indicates the cleavage site). The crystal structure of its active site mutant D83A bound to cognate DNA has been determined at 1.7 Å resolution. Base-specific contacts between MunI and DNA occur exclusively in the major groove. While DNA-binding sites of most other restriction enzymes are comprised o...
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The crystal structure of EcoRV endonuclease has been determined at 2.5 A resolution and that of its complexes with the cognate DNA decamer GGGATATCCC (recognition sequence underlined) and the non-cognate DNA octamer CGAGCTCG at 3.0 A resolution. Two octamer duplexes of the non-cognate DNA, stacked end-to-end, are bound to the dimeric enzyme in B-DNA-like conformations. The protein--DNA interact...
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برای پردازش سیگنال آنالوگ در شبکه های عصبی ، معمولا نیاز به یک واحد حافظه آنالوگ احساس میشود که بدون احتیاج به a/d وd/a بتواند بطور قابل انعطاف و مطمئن اطلاعات آنالوگ را در خود ذخیره کند. این واحد حافظه باید دارای دقت کافی ، سرعت بالا ، توان تلفاتی کم و سایز کوچک باشد و همچنین اطلاعات را برای زمان کافی در خود نگهدارد. برای پیاده سازی سیستمی که همه این قابلیتها را در خود داشته باشد، کوشش...
15 صفحه اولAn investigation of neutron direct damages at energies of 0.1-2 MeV on the DNA molecules with atomic structure deduced using Geant4 toolkit
This study proposes a method to estimate RBE of fast neutrons using Monte Carlo simulations. This approach is based on the combination of an atomic resolution DNA geometrical model and Monte Carlo simulations for tracking particles. Atomic positions were extracted from the Protein Data Bank. The GEANT4 code was used for tracking the secondary particles generated by fast neutrons during their in...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2019
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkz1195